Fast and accurate arbitrary waveform generators (AWG) for generating shaped pulses in EPR have been commercially available for over a decade now but, while using chirp pulses as inversion pulses in pulsed electron double resonance (PELDOR) experiments has become common, their usage for generating broadband phase-sensitive transverse magnetization has remained less frequent and is not widely adopted within the community. Here we give a detailed insight into optimization procedures and instrumental challenges when using chirped pulses for broadband Fourier transform (FT) detection of electron spin echo signals, particularly the two-dimensional frequency¬ correlated single frequency technique for refocusing (SIFTER) experiment. To better understand the influence of chirped pulses on the generation of broadband transverse magnetization we investigated the phase and amplitude of chirped echoes for different time-bandwidth products and with varying numbers of refocusing pulses, particularly under the influence of B_1 inhomogeneity.
Following our optimization procedures, we were able to perform orientation selective SIFTER measurements using rigid nitroxide spin labels on an RNA duplex. Finally, we also demonstrate first experiments with two novel SIFTER pulse sequences which could be of interest for the detection of shorter or longer distances, respectively.

Raw data sets of 2D SIFTER experiments in .mat format. Matlab scripts for calculation of relative pulse lenghts to refocus parabolic phase shift with chirped pulses.